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Image Search Results


Journal: bioRxiv

Article Title: A Novel Single Vector Intersectional AAV Strategy for Interrogating Cellular Diversity and Brain Function

doi: 10.1101/2023.02.07.527312

Figure Lengend Snippet:

Article Snippet: To begin building ConVERGD, we synthesized an AAV-backbone plasmid (pAAV-hSyn-FLEx(FRT)-eGFP-W3SL) (Azenta Life Sciences) containing the minimal human synapsin promoter (hSyn), an antisense eGFP coding region within a Flp-dependent FLEx-switch, the W3SL (WPRE3 (‘W’) followed by the SV40 late polyadenylation sequence with an upstream element (‘SL’)) 3’ posttranscriptional regulatory cassette, and robust multiple-cloning sites that provided easy component exchange.

Techniques: Cloning, Cell Culture, Sequencing, Software